Research Facilities
Biophysical Spectroscopy Center
- Electron Paramagnetic Resonance (EPR)
- Fluorescence and Luminescence Resonance Energy Transfer (FRET and LRET)
- Steady-state (Eclipse, Gemini)
- Time-resolved (Fargoland, Fluorescence Lifetime Plate Reader)
- Fluorescence microscopy (Nikon, FLIM)
- Time-resolved Phosphorescence Anisotropy (TPA)
- Collaborator: Gianluigi Veglia
- Solid-Phase Peptide Synthesis
- Collaborator: Christine Karim
- Purification/HPLC/MS
- Peptide Spin Labeling (Karim et al., Nature Protocols, 2008)
Techniques
Computation
- Data acquisition and analysis
- Molecular dynamics simulations
- Bengt Svensson, Michel Espinoza-Fonseca
- Collaborator: Jonathan Sachs
- Supercomputer simulations of spectroscopy
- Basic Sciences Computing Laboratory
- Spectroscopy and x-ray diffraction on labeled protein crystals
- Collaborator: John Lee
- Correlate biochemical and molecular dynamics
- Transient kinetics using laser flash photolysis
- Transient Time-resolved FRET
- ATPase assays
- Muscle mechanics
- Effects of aging, muscular dystrophy
- Collaborator: Dawn Lowe
- Molecular Biology:
- Site-directed mutagenesis
- site-directed labeling
- fluorescent fusion proteins
- Cell culture: bacteria, Dictyostelium, insect, yeast, mammalian